Authors :
Presenting Author: Karla Vazquez Olaya, – Benemerita Universidad Autonoma de Puebla
Carmen Cortes, PhD – Benemerita Universidad Autonoma de Puebla
Jose R. Eguibar, PhD – Benemerita Universidad Autonoma de Puebla
Rationale:
The taiep rat is a tubulin mutant, and its name is an acronym for the progressive motor signs it exhibits: tremor, ataxia, immobility, epilepsy (absence seizures), and paralysis. Absence seizures are characterized by spike-wave discharges (SWDs) in the electroencephalogram (EEG). It has been postulated that SWDs are modulated by sex hormones. The SWDs in the taiep rat exhibit sexual dimorphism, appearing in males at 3 months and in females at 6 months. The objective of this study was to evaluate the effect of medroxyprogesterone acetate (MPA) on SWDs in female taiep rats. Methods:
A bilateral ovariectomy (Ovx) was performed on 6-month-old female taiep rats, and electrodes were implanted for EEG recording. Three 24-hour EEG recordings were conducted: the first as a control, and two following intramuscular administration of MPA at doses of 5 and 10 mg/kg, with a 72-hour interval between each. Results:
The results show that MPA administration reduced the frequency of SWDs by 52% and 25.5% with the 5 and 10 mg/kg doses, respectively. The total durations of SWDs also decreased by 48% and 40%, respectively. However, the latency to the first SWD only increased with the administration of 5 mg/kg of MPA.Conclusions:
In conclusion, MPA administration reduces the frequency and duration of SWDs and increases their latency in adult Ovx female taiep rats.
Funding:
Mainly funded by PRONACES-CONACYT 194171, VIEP-BUAP 2024 through the Academic Body of Neuroendocrinology (BUAP-CA-288) and by CAMELICE-2024.