Altered Development of Brain Glucose Metabolism in a Rat Model of Cortical Malformation
Abstract number :
1.072
Submission category :
1. Basic Mechanisms / 1F. Other
Year :
2021
Submission ID :
1826412
Source :
www.aesnet.org
Presentation date :
12/9/2021 12:00:00 PM
Published date :
Nov 22, 2021, 06:54 AM
Authors :
Min-Jee Kim, MD, PhD - Asan Medical Center Children’s Hospital, Ulsan University College of Medicine; Mi-Sun Yum – Department of Pediatrics – Asan Medical Center Children’s Hospital, Ulsan University College of Medicine; Minyoung Lee – Department of Pediatrics – Asan Medical Center Children’s Hospital, Ulsan University College of Medicine; Eun-Jin Kim – Department of Pediatrics – Asan Medical Center Children’s Hospital, Ulsan University College of Medicine; Tae-Sung Ko – Department of Pediatrics – Asan Medical Center Children’s Hospital, Ulsan University College of Medicine
Rationale: Malformations of cortical development (MCD) is associated with a wide range of developmental delay and drug resistant epilepsy in children. By using 18-fluorodeoxyglucose positron emission tomography/magnetic resonance (FDG-PET/MR) imaging, we tried to investigate the developmental change of brain metabolism in a rat model of methylazoxymethanol (MAM)-induced MCD.
Methods: A total of 25 infant rats with prenatal exposure to MAM and 13 age matched controls with prenatal saline exposure were used. After acquisition of baseline FDG-PET/MR images at day 13 (P13), they were randomly divided into two groups; spasms and non-spasms. At P15, spasms group were triggered by intraperitoneal injection of N-methyl-d-aspartic acid (NMDA) and 18-FDG was injected 30 minutes after NMDA injection for PET/MR. The standardized uptake value (SUV) of the four Region of Interests (ROIs; striatum, cortex, thalamus, hippocampus) were measured and normalized using cerebellum SUV. These normalized values of SUVs in each ROIs were compared between MCD rats and controls and among each time points within each group.
Results: At P13 and P15, MCD rats showed significantly decreased glucose metabolism in all ROIs compared to controls (Mann-Whitney test; P13, p < 0.05
Basic Mechanisms