EVALUATION OF OXIDATIVE DNA DAMAGE IN CHILDREN WITH BRAIN DAMAGE UTILIZING 8-HYDROXYDEOXYGUANOSINE
Abstract number :
2.172
Submission category :
Year :
2005
Submission ID :
5476
Source :
www.aesnet.org
Presentation date :
12/3/2005 12:00:00 AM
Published date :
Dec 2, 2005, 06:00 AM
Authors :
1Miho Fukuda, 1Hitoshi Yamamoto, 2Hiroshi Yamauchi, 2Yoshito Aminaka, and 1Yasushi Koitabashi
To validate the utility of urinary 8-hydroxydeoxyguanosine (8-OHdG) as a marker of oxidative stress in children with brain diseases. Urinary levels of 8-OHdG were measured in 25 children with various forms of acute brain diseases (SE, HIE, and CNS infections), 11 children with chronic epilepsy, 51 healthy children and 248 healthy adults. We compared urinary 8-OHdG levels between children with acute brain diseases, children with chronic epilepsy, healthy children (unpaired t-test). We also determined the correlation between urinary and CSF levels of 8-OHdG in 12 children with acute brain diseases. The level of urinary 8-OHdG in children with HIE was 4 times higher than that of normal healthy children (p[lt]0.05). The level of urinary 8-OHdG in normal healthy children was approximately 20% higher than that of healthy adults (p[lt]0.03). The level of CSF 8-OHdG in healthy children was 4.14[plusmn]2.06pg/ml (n=19). The level of CSF 8-OHdG in children with SE, HIE and CNS infections were significantly higher than control group (p[lt]0.05, p[lt]0.005, p[lt]0.05). The level of urinary and CSF 8-OHdG in children with epilepsy did not differ from that of normal healthy children (p=0.28, p=0.46). Temporary changes in urinary levels of 8-OHdG was demonstrated over the course of disease in a patient with human herpes virus 6 encephalopathy. A significant positive correlation between levels of urinary and CSF 8-OHdG were noted in children with acute brain diseases (Pearson correlation; r=0.76, p[lt]0.001). CSF 8-OHdG reflects active oxidative stress induced brain injury in children more closely than urinary 8-OHdG. On the other hand, the urinary 8-OHdG was shown to be useful as a clinical marker which is simple and non-invasive compared with CSF 8-OHdG. Urinary 8-OHdG appears to be a useful marker of disease activity in childhood nervous disorders in which oxidative stress plays an important levels of 8-OHdG and can be correlated with clinical progress. The time course of 8-OHdG levels during illness and convalescence in a large range of disorders are needed.