Optimizing Tet1 siRNA in Primary Neuronal Cultures to Silence Gene Expression
Abstract number :
1.419
Submission category :
1. Basic Mechanisms / 1B. Epileptogenesis of genetic epilepsies
Year :
2022
Submission ID :
2232863
Source :
www.aesnet.org
Presentation date :
12/3/2022 12:00:00 PM
Published date :
Nov 22, 2022, 05:28 AM
Authors :
Forest Nandjou, BS – Alabama A&M University; Adam Aldaher, BS – University of Alabama at Birmingham; Rudhab Bahabry, MD – University of Alabama at Birmingham; Farah Lubin, PhD – University of Alabama at Birmingham
Rationale: Recent studies have revealed the vital importance of epigenetic mechanisms in diverse biological processes and diseases. Accordingly, the DNA hydroxymethylation (5-hmC), induced by Ten-eleven translocation (Tet) enzymes, has been shown to be critical for the regulation of neuronal function, synaptic plasticity, and gene expression in the brain.
Methods: Recent studies have revealed the vital importance of epigenetic mechanisms in diverse biological processes and diseases. Accordingly, the DNA hydroxymethylation (5-hmC), induced by Ten-eleven translocation (Tet) enzymes, has been shown to be critical for the regulation of neuronal function, synaptic plasticity, and gene expression in the brain.
Results: There were no changes in the expression levels of cultures not treated with jetPEI (untreated, treated with red non-targeting siRNA, Tet1 siRNA, and GAPH siRNA). We expect an improved cellular uptake of Tet1 and a reduction in gene expression with the use of jetPEI in our neuronal cultures.
Conclusions: Future studies are needed to evaluate Tet1 siRNA in vivo using jetPEI as a transfection reagent.
Funding: American Epilepsy Association, Blazer Brain UAB, Lubin’s Lab.
Basic Mechanisms