P-glycoprotein is overexpressed in amygdala and hippocampus during electrical amygdala kindling process
Abstract number :
1.414
Submission category :
1. Basic Mechanisms / 1A. Epileptogenesis of acquired epilepsies
Year :
2022
Submission ID :
2232894
Source :
www.aesnet.org
Presentation date :
12/3/2022 12:00:00 PM
Published date :
Nov 22, 2022, 05:28 AM
Authors :
Daniel Fonseca-Barriendos, MD, MSc – Center for Research and Advanced Studies; José Luis Castañeda-Cabral, PhD – epartamento de Biología Celular y Molecular – Universidad de Guadalajara; Walter Besio, PhD – Department of Electrical, Computer, and Biomedical Engineering – University of Rhode Island; Sandra Orózco-Suárez, PhD – Unidad de Investigación Médica en Enfermedades Neurológicas – Hospital de Especialidades "Dr. Bernardo Sepúlveda"; Alejandro Valdés-Cruz, PhD – División de Neurociencias – Instituto Nacional de Psiquiatría "Ramón de la Fuente Muñíz"; Luisa Rocha, PhD – Pharmacobiology – Center for Research and Advanced Studies
Rationale: P-glycoprotein (P-gp) is an ATP-dependent drug efflux pump that acts as a xenobiotic extruder and its overexpression is associated to drug resistant epilepsy. Moreover, P-gp overexpression is detected after an acute seizure and has been associated to neuronal hyperexcitability. Therefore, we aim to determine if P-gp expression is progressively increased through epileptogenesis induced by electrical amygdala kindling.
Methods: Male Wistar rats previously implanted in right basolateral amygdala were used (n=5 per group). Briefly, kindled group was stimulated daily until 3 consecutive stage V seizures were induced. Stage III group was stimulated until one stage III seizure was elicited. Stage I group in which only one discharge was evoked. Naïve animals weren’t implanted nor stimulated. Amygdala and hippocampi (ipsi- and contralateral) were collected 24 h after the last electrical stimulation or manipulation. P-gp expression was evaluated by Western blot.
Results: Stage I group showed P-gp overexpression in right hippocampus (86%, p< 0.001 vs. naïve). Stage III group presented P-gp overexpression in right amygdala (77.79%, p< 0.05 vs. naïve), and both hippocampi (58.95%, p< 0.05 and 57.66%, p< 0.05 vs. naïve, right and left respectively). Kindled group had P-gp overexpression in right amygdala (89.95%, p< 0.01 vs. naïve) and both hippocampi (92.20%, p< 0.001 and 91.73%, p< 0.01 vs. naïve, right and left respectively).
Basic Mechanisms