Sleep-seizure associations in cortical EEG in a transgenic mouse model of progressive epilepsy: implications for morphological changes in cortico-limbic and brainstem structures
Abstract number :
1.473
Submission category :
3. Neurophysiology / 3A. Video EEG Epilepsy-Monitoring
Year :
2022
Submission ID :
2232890
Source :
www.aesnet.org
Presentation date :
12/3/2022 12:00:00 PM
Published date :
Nov 22, 2022, 05:28 AM
Authors :
Amanda Zwick, BS – Florida Atlantic University; Anastasia Tyulmenkova, BS – Florida Atlantic University; Giovanny Garcia-Mendoza, BS – Florida Atlantic University; Sebastien Nicolas, BS – Florida Atlantic University; Ceylan Isgor, PhD – Principle Investigator, Biomedical Sciences, Florida Atlantic University
This is a Late Breaking abstract
Rationale: Sleep-seizure cortical electroencephalography (EEG) is evaluated to track severity of upcoming seizures and death risk in a mouse model of adult-onset generalized tonic clonic seizures (GTCSs). The mice overexpress the brain derived neurotrophic factor (BDNF) in the forebrain (termed TgBDNF) and develop GTCSs in response to tail lifts/cage agitation in adulthood.
Methods: TgBDNF and litter-matched wild type (WT) controls were implanted with subdural cortical electrodes to monitor sleep and seizure video/EEG from early to advanced stages of convulsive seizures across 7 weeks.
Results: Seizures became more severe as evidenced by increased duration of postictal generalized EEG suppression (PGES) associated with loss of posture/consciousness. Some TgBDNF mice expired following a long period of PGES. A deficit in rapid eye movement (REM) state was observed in TgBDNF mice in sleep/awake transition (i.e., late sleep). In contrast slow-wave sleep (SWS) bout duration and percentage was higher in late sleep in convulsive TgBDNF compared to WT mice. Less frequent REM bouts in late sleep was associated with enhanced consciousness impairment in GTCSs by longer duration of PGES and cumulative duration of spike wave discharges accompanied with brief behavioral arrest during seizure recovery. Increased percentage of SWS in late versus early sleep (i.e., awake/sleep transition) was also linked to longer PGES duration.
Conclusions: Striking decrease in total number of gigantocellular neurons of the brainstem nucleus pontis oralis along with increase in volume of the anterior cingulate cortex and dorsal dentate gyrus were observed in severely convulsive TgBDNF mice, signaling a deficit in cortical arousal and autonomic control functions.
Funding: NIH/NINDS Grant NS 115049
Neurophysiology