Study of the anti-inflammatory effect of cannabidiol and in the control of seizures induced with kainic acid in immature rats.
Abstract number :
1.418
Submission category :
7. Anti-seizure Medications / 7A. Animal Studies
Year :
2022
Submission ID :
2232937
Source :
www.aesnet.org
Presentation date :
12/3/2022 12:00:00 PM
Published date :
Nov 22, 2022, 05:28 AM
Authors :
Omar García, PhD – ENCB-IPN; Angélica Vega, PhD – Physiology – UNAM; Sergio Meza, PhD – Biochemistry – ENCB-IPN; Sandra Orozco, PhD – Neurological Diseases Medical Research Unit – IMSS
Rationale: To evaluate the anti-inflammatory effect of cannabidiol on cyclooxygenase-2 (COX-2), interleukin 1-β (IL 1-β), and tumor necrosis factor α (TNF-α) expression induced by lipopolysaccharide (LPS) administration and on seizure susceptibility in immature rats.
Methods: Male rats, Sprague-Dawley strain of 10 days post natal age (PN), were administered a dose of LPS of 1 mg/kg i.p for 5 days with an n=40, the following groups were performed: Saline (SHAM) (0.9% NaCl v.o.), Celecoxib (CLBX) 20 mg/kg/v.o. and cannabidiol (CBD) 20 mg/kg/v.o. They were administered 2 h post LPS, every 24 h for 5 days, at 14 PN seizure susceptibility was evaluated with a sub-convulsive dose of kainic acid (AK), i.p. 1.5 mg/kg. By Western blot, protein levels of COX-2, IL 1-β and TNF- α were analyzed in hippocampal tissue at 6, 12, and 24 h post AK.
Results: The CBD experimental group showed significant decrease in the expression of COX-2 and proinflammatory cytokines IL 1-β and TNF- α, similar to the CLBX group at 6 (p< 0.001), 12 (p< 0.001) and 24 (p< 0.001) h compared to the LPS group, with a maximum peak at 12 hours. CBD increased seizure activity latencies with 60% of rats presenting severe seizures and 15% status epilepticus, similar to the CLBX group with 80% severe seizures and 25% SE, compared to the LPS-treated group (100% severe seizures and 80% SE).
Anti-seizure Medications