Testosterone enanthate administration increases spike–wave discharges in male taiep rats
Abstract number :
3.457
Submission category :
1. Basic Mechanisms / 1E. Models
Year :
2025
Submission ID :
1448
Source :
www.aesnet.org
Presentation date :
12/8/2025 12:00:00 AM
Published date :
Authors :
Presenting Author: Ruben Castillo Lazcano, PhD Student – Universidad Autónoma de Tlaxcala
Carmen Cortes, PhD – Benemérita Universidad Autónoma de Puebla
Araceli Ugarte, MS – Casa Azul, Zaguán Blanco entre Roble y C
José R. Eguibar, PhD – Benemérita Universidad Autónoma de Puebla
Rationale: Absence seizures are characterized by spike–wave discharges (SWDs) in electroencephalographic recordings. The taiep rat carries a mutation in the Tubb4a gene that produces hypomyelination with atrophy of the basal ganglia and cerebellum (H-ABC). Male taiep rats show SWDs at an early age. In our laboratory we have demonstrated that males exhibit a higher SWD frequency than females, and that neonatal orchiectomy significantly reduces SWD frequency. To evaluate the effect of testosterone enanthate (TE) on SWDs in orchiectomized male taiep rats.
Methods: Male taiep rats were orchiectomized at postnatal day 165 ± 2. One week later they were implanted for electroencephalographic, electrooculographic, and electromyographic recordings. Two EEG sessions were performed: (1) a control session after subcutaneous (s.c.) administration of olive oil (vehicle, 1 mL/kg), and (2) a session after s.c. administration of testosterone enanthate (25 mg/kg). The total frequency of SWDs was quantified.
Results: Testosterone enanthate significantly increased the total frequency of SWDs compared with the control session (Student’s t-test = 2.70, p < 0.05).
Basic Mechanisms